Journal: Journal of Traditional and Complementary Medicine
Article Title: Polysaccharides derived from alkali-extracted vinegar-baked Radix Bupleuri suppress hyperimmune T lymphocytes and ameliorate skin graft rejection
doi: 10.1016/j.jtcme.2024.11.006
Figure Lengend Snippet: The effect of KVBCP3 and KVBCP4 on immune cytokines and Treg cells of mice with skin allograft rejection. (A) The secretion of IL-2 in serum was determined by Elisa assay (n = 6). (B) The secretion of IL-4 in serum was determined by Elisa assay (n = 6). (C) Representative flow cytometry plots of CD4 + CD25 + Foxp3 + Treg cells after treatment with KVBCPs at the 14th day; (D) The change of the proportions of CD4 + CD25 + Foxp3 + Treg cells after treatment with KVBCPs at the 14th day (n = 3). (E) Representative flow cytometry plots of CD4 + IL-17A + cells after treatment with KVBCPs at the 14th day; (F) The change of the proportions of CD4 + IL-17A + cells after treatment with KVBCPs at the 14th day (n = 3). ∗ P < 0.05, ∗∗ P < 0.01 vs Model group, one way ANOVA.
Article Snippet: Cell stimulation and Protein Transport Inhibitor Kit (Lot: AK22752), FITC Anti-Mouse CD4 Antibody (Lot: AF21880), PE Anti-Mouse IL-17A Antibody (Lot: AF17079) were purchased from Elabscience (Wuhan, China).
Techniques: Enzyme-linked Immunosorbent Assay, Flow Cytometry